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. 2012 Aug 3;7(8):e41401. doi: 10.1371/journal.pone.0041401

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) CHA59, Saos-2, and HuO9 monolayers generated spheres in FBS-free, SS media. Phase contrast images taken with a 40× objective. (B) CHA59 spheres formed colonies in both FBS-A (Fetal Bovine Serum-Agarose) and SS-A (KnockOut Serum Replacement-Agarose). Images taken with a 10× objective. C) CHA59 spheres demonstrated significantly higher clonogenicity as compared with monolayers in FBS-A and SS-A, * p<0.05. (D) CHA59 Spheres demonstrated higher tumorigenicity than monolayers. 10 NOD/SCID mice injected with cells per group. A xenograft of ≥150 mg was counted positive for take rate.