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. 2012 Aug 3;7(8):e42193. doi: 10.1371/journal.pone.0042193

Figure 4. BCL6 is a target of miR-205.

Figure 4

A: Effect of the analysed miRNAs on the empty vector. Cells co-transfected with the miRNA expression vectors, vector control and the empty reporter vector ware tested in luciferase assays. The diagram represents five independent experiments carried out in duplicate with the standard error indicated. The Renilla luciferase activity was used for normalization and firefly luciferase activity of control transfected cells was set to 100%. B: Regulation of the luciferase activity of the reporter construct containing the BCL6 3′UTR by several miRNAs predicted to target the 3′UTR. The bars represent four different experiments done in duplicate. C: Schematic overview of the predicted binding site of miR-205 in the 3′UTR of BCL6. The sequence in the 3′UTR mutated by site-directed-mutagenesis is indicated. D: Influence or miR-205 on the luciferase activity of the empty vector, the wt-3′UTR and the mutated BCL6 3′UTR. The diagram shows the results of four independent experiments carried out in duplicate. E: Transfection of miR-205 reduces the BCL6 protein in SUP-T1 cells. SUP-T1 cells transfected with miR-205 or a vector control were analysed in a Western blot with BCL6- and ß-actin- specific antibodies. The signals for ß-actin served as a loading control.