Table 1. Origin and characteristics of human cell line models under investigation.
| Cell line | Cell type | Origin | Doubling time [h]1 | BCR-ABL-copy no/type | Cytogenetics | Used as model for |
| NHDF | normal human dermal fibroblasts, primary | juvenile foreskin, from healthy donor | ∼72 | none | 46,XY | normal cells |
| UROtsa | human urothelial | normal urothelial cells immortalized with SV40 | ∼63 | none | 46,XX | normal cells |
| HL-60 | human AML-M2 | PB of a patient with AML-M2 | ∼40 | none | 82–88<4n>XX,−X,−X,−8,−8,−16,−17,−17,+18,+22,+2mar, ins(1;8)(p?31;q24hsr)x2,der(5)t(5;17)(q11;q11)x2,add(6) (q27)x2,der(9)del(9)(p13)t(9;14)(q?22;q?22)x2,der(14) t(9;14)(q?22;q?22)x2,der(16)t(16;17)(q22;q22)x1-2, add(18)(q21) - sideline add(18)(q21) - sideline with: −2,−5,−15,del(11)(q23.1q23.2) - c-myc amplicons present in der(1) and in both markers (DSMZ) | bcr-abl-negative leukemia |
| U937p210BCR-ABL/c6 | human histiocytic lymphoma with recombinant p210BCR-ABL gene | pleural effusion of a patient with generalized histiocytic lymphoma, stable transfected with p210BCR-ABL cDNA construct under Tet-On promoter control [17] | 37 (ON)2 | 1/(b3a2) | 47∼94,X,−Y,+1,t(1;6;1),t(1;16),+2,t(2;5),+4,del(4q),+5, del(5p),der(6)t(6;19)del(6p),+7,+7,t(9;17),−10,t(10;13), del(11q),+13,add(13q),t(14;15),+15,+15,+16,+17[cp10] | CML-CP |
| LAMA-84 | human CML (BC) | PB of a patient with CML BC | ∼30 | 4/(b3a2) | 73/74(69–77)<3n>XX,−X,+1,−2,+5,+6,del(7)(p15),+8, der(9)t(9;22)(q34;q11)x2,i(11q),+13,add(13)(q33),−14,+17,+17,del(17)(p12),−18,+22, der(22)t(9;22)(q34;q11)x4,+mar, (DSMZ) | CML-BC |
| K562 | human CML (BC) | pleural effusion of a patient with CML in BC | ∼34 | 11/(b3a2) | 61–68<3n>XX,−X,−3,+7,−13,−18,+3mar,del(9)(p11/13), der(14)t(14;?)(p11;?),der(17)t(17;?)(p11/13;?),der(?18) t(15;?18)(q21;?q12),del(X)(p22) (DSMZ) | CML-BC |
Abbreviations: PB, peripheral blood; CML, chronic myeloid leukemia; CP, chronic phase; BC, blast crisis; no, number; AML-M2, acute myeloid leukemia M2; DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
1
doubling time of subconfluent cells when 3×105 cells were seeded in 100 mm cell culture dishes.
2
p210BCR-ABL Tet-ON promoter induction with Doxycycline [1 µg/ml].