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. 2012 Aug 3;7(8):e42302. doi: 10.1371/journal.pone.0042302

Figure 2. ZFX BAC transgenic human embryonic stem cells.

Figure 2

A. Normalized ZFX expression levels from G418-resistant hESC clones with the original clone names shown on the x-axis. Three clones were renamed ZFXOver1, ZFXOver2 and ZFXNormal were selected for further analysis. ZFXNormal showed normal levels of ZFX but had undergone the same clonal and selection steps as ZFXOver clones. B. ZFX and actin protein levels determined by Western blot analysis of ZFXOver clones and controls. C. Oct4 (purple), SSEA-4 (green), Tra1-81 (red) and DAPI (blue) on ZFXOver clones and controls. Scale bar  = 500 µm (low) or 100 µm on the high magnification images.