Figure 4. The entire Tween-20 molecule is required to block nuclear tRNA export in HeLa cells.

HeLa cells were treated with 50–300 µM (A) lauric acid (B), SPAN20 and (C) polyethylene glycol for 4 h in serum-free DMEM. The cells were fixed and FISH was used to monitor the location of tRNA^Lys. The cells were stained with DAPI to visualize the nucleus. Scale bar represents 10 µm.