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. 2012 Aug 3;7(8):e42501. doi: 10.1371/journal.pone.0042501

Figure 5. The nuclear tRNA and mRNA export defect caused by Tween-20 is reversible.

Figure 5

HeLa cells were left in serum-free media (panels A and B, Untreated) or treated with 150 µM Tween-20 for 4 h in serum-free DMEM. The cells were then kept in the presence of Tween-20 (second panel, Tween-20), or washed and placed in fresh serum-free DMEM for 1 h (third panel, Wash). The cells were then fixed and processed for FISH to monitor the distribution of (A) tRNALys or (B) mRNA. (C) Tween-20 treated cells were washed and placed in serum-free DMEM and incubated for the times indicated. The location of tRNALys was monitored by FISH. The cells were DAPI stained to visualize the nucleus. Scale bar represents 10 µm.