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. 2012 Aug 3;7(8):e42501. doi: 10.1371/journal.pone.0042501

Figure 10. Tween-20 causes nuclear accumulation of NTF2.

Figure 10

HeLa cells were incubated in serum-free DMEM without (Untreated) or with 150 µM Tween-20 for 4 h (Tween-20), or in serum-free DMEM with 150 µM Tween-20 for 4 h and then washed and incubated in serum-free DMEM without Tween-20 (washed) for 1 h. The cells were washed and fixed in 1× PBS containing 4% paraformaldehyde, and the distribution of NTF2 was monitored by immunofluorescence microscopy. The cells were stained with DAPI to visualize the nucleus. Scale bar represents 10 µm.