Figure 1. Alignment of HvHMA2 with various plant P_1B-ATPases showing the predicted TMs.

Swissprot TM predictions for AtHMA2 are indicated, except TM6 was extended according to the SOSUI prediction. Shaded boxes indicate motifs conserved in P-type ATPases, other boxes indicate motifs in P_1B-ATPase subgroup, and asterisks indicate residues conserved in P_1B-2-ATPases that may co-ordinate the metal ion during transmembrane transport and contribute to ion specificity. Conserved (upper-case) and semi-conserved (lower-case) amino acids are indicated beneath the alignment.