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. 2012 Aug 3;7(8):e42561. doi: 10.1371/journal.pone.0042561

Figure 4. ATPase and ADPase activities of MTB-PPX1 and Rv1026.

Figure 4

Reaction mixtures (100 μl) containing 1 μg protein (MTB-PPX1, Rv1026, E. coli GPP or E. coli PPX), 1 mM ATP or ADP, 4 mM (NH4)2SO4, in Tris-HCl buffer (50 mM, pH 7.4), with or without 1 mM MnCl2 or MgCl2; were incubated at 30°C for 0, 60 or 120 minutes (as indicated). Product mixtures were analyzed using anion exchange chromatography, with chromatograms shown in Panels A–F. Panel A : MTB-PPX1 + ATP; no added metal ion (blue line), with 1 mM MgCl2 (green line), with 1 mM MnCl2 (black line); incubated for 60 minutes. Panel B: Rv1026 + ATP (as for Panel A). Panel C: E. coli GPP (EC-GPP) + ATP + MnCl2: 0 minutes incubation (blue line), 60 minutes incubation (green line). Panel D: E. coli PPX (EC-PPX) + ATP + MnCl2 (as for Panel C). Panel E: MTB-PPX1 + ADP + MnCl2; 0 minutes (blue line), 60 minutes (green line), 120 minutes (black line). Panel F: Rv1026 + ADP + MnCl2 (as for Panel E).