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. 2012 Aug 3;7(8):e42653. doi: 10.1371/journal.pone.0042653

Figure 7. Genotypic and phenotypic changes in the derivative clones from cancer-stromal fusion.

Figure 7

Genotypic and phenotypic parameters of the first 9 clones of the RL-1 and HPS-15 fusion hybrids were compared to those of the first 12 clones from control cloning. Compared to RL-1 clones, all the derivative clones lost Y chromosomes (A versus B). Detected by Western blotting, some of the derivative clones showed persistent AR expression even under androgen-deprivaton (C versus D). In these studies, cells were cultured for 48 hours in regular culture medium (C), androgen deprivation medium (−), and androgen deprivation medium containing 5 nM R1881 (+). The derivative clones were detected to express increased levels of PSA, even during androgen-deprivation (E versus F). When growth rate was assayed by MTT conversion, clones derived from cancer-stromal fusion displayed accelerated growth in androgen-independent fashion (G versus H). Data represent the mean of triplicate assays. For all the data points, standard deviation was less than 5% of the mean and is not shown.