Figure 2. Effect of Tβ4 on anti-oxidative enzymes under oxidative stress in cardiomyocytes.

(A) Relative fold change in the mRNA expression of Mn-SOD, (B) Cu/Zn-SOD and (C) Catalase, in cardiomyocytes treated with H₂O₂ in presence and absence of Tβ4. Data represent the means ± SE of at least three separate experiments. (D) Western blots showing the protein expression of Mn-SOD, Cu/Zn-SOD and catalase at 12 h and 24 h, respectively. GAPDH was used as internal loading control for the experiment. (E) Graph shows the relative fold change in the protein expression of Mn-SOD, Cu/Zn-SOD and catalase, respectively by densitometry. Data represent means ± SEM from 3 individual experiments. * denotes p<0.05 compared to controls while ^# denotes p<0.05 compared to the H₂O₂-treated group.