Figure 3. Effect of Tβ4 on pro-and anti-apoptotic proteins under oxidative stress in cardiomyocytes.

Relative fold change mRNA expression of (A) Caspase-3, (B) Bax and (C) Bcl₂. Data represent the means ± SE of at least three separate experiments. (D) Protein expression of Caspase-3, Bax and Bcl₂ at 12 h and 24 h, respectively. GAPDH was used as loading control for the experiment. (E) Graph shows the relative fold change in the protein expression of Bax, Bcl₂ and caspase-3, respectively by densitometry. Data represent means ± SEM from 3 individual experiments. * denotes p<0.05 compared to controls while ^# denotes p<0.05 compared to the H₂O₂-treated group and ^@ means p = ns compare to the H₂O₂-treated group.