Table 1. Image intensities (Arbitrary Units) showing the fluorescence intensities in cardiomyocytes upon staining with DCF-DA, DHE, DAF-2DA and MitoTracker Red.
S. No. | Staining | Control | Tβ4 | H2O2 | H2O2+Tβ4 |
1 | DCF-DA | 339±29 | 213±43* | 792±53* | 273±32# |
2 | DHE | 592±71 | 602±68 | 904±50* | 711±62# |
3 | DAF-2DA | 414±39 | 184±37* | 1311±92* | 375±29# |
4 | MitoTracker Red | 361±38 | 370±47 | 237±29* | 349±24# |
Data acquired from at least 15 fields taken from 3–4 different confocal images of the same treatment group and were quantified by using ImageJ Software.
denotes p<0.05 compared to controls while # denotes p<0.05 compared to the H2O2-treated group.