Figure 5. Attenuated GvHD using RORC^−/− Tconv cells is mediated by CD4⁺ T cells.

(A) Lethally irradiated B6D2 F1 mice were injected with 3 × 10⁶ TCD BM. In addition to BM one group received 2 × 10⁶ RORC^−/− CD4⁺ T cells and 2 × 10⁶ WT CD8⁺ T cells, one group received 2 × 10⁶ WT CD4⁺ T cells and 2 × 10⁶ RORC^−/− CD8⁺ T cells, and a final group received only BM cells. Recipients of RORC^−/− CD4⁺ T cells with WT CD8⁺ Tcells showed less GvHD reaching statistical significance by day 15 post transplantation. n= 11 recipient mice receiving RORC^−/− CD4⁺ T cells and WT CD8⁺ T cells, n=5 for recipient mice receiving WT CD4⁺ T cells and RORC^−/− CD8⁺ T cells, n=4 for bone marrow only. Data are combined from 2 individual experiments *p<0.05. (B) 10 days post transplantation the livers of RORC^−/− CD4⁺, WT CD8⁺ T cell or WT CD4⁺, RORC^−/− CD8⁺ T cell recipient mice were harvested and T cells isolated. Data are representative from 3 WT CD4, RORC^−/− CD8⁺ recipients and 4 RORC^−/− CD4⁺, WT CD8⁺ recipients.