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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Immunol. 2012 Jul 11;189(4):1780–1791. doi: 10.4049/jimmunol.1103768

FIGURE 7. The effect of IL2-IC on Treg subsets.

FIGURE 7

(A–D) WT B6 mice received 3 daily injections of IL2-IC complex. 3, 5, or 7 days after the last injection, splenic Tregs were evaluated. Control represents mice that were injected with PBS and were analyzed only 3 days after the last injection. Representative FACS profiles of the indicated markers 3 days after the last injection of IL2-IC (A) and time course of Treg numbers (B) of the indicated populations. (C) Expression of Ki67 and Bcl-2 3 days after the last injection of IL2-IC. (D) Time course for the expression of the indicated markers. Data are from at least 3 mice per group per time point. (E) Splenic Klrg1+ or Klrg1− Tregs (1 × 105) from the Foxp3/RFP reporter mice were injected with splenic conventional CD4+ T cells (1 × 106) from the Foxp3/GFP reporter mice at the ratio of 1:10 into TCRα−/− mice. One day later the recipients received 3 daily injections of IL2-IC. 6 days after the cell transfer, the indicated cell populations from the spleen were analyzed for the expression of RFP and Klrg1. Data are 4–5 mice/group where each point on the graph represents an individual mouse