Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Nat Med. 2012 Jun;18(6):934–942. doi: 10.1038/nm.2744

Figure 1. Liver-specific deletion Hdac3 in adult mice causes quick onset of hepatosteatosis that is distinct from high-fat diet-induced fatty liver.

Figure 1

(a) Western blot of total liver lysates at 1-week after AAV injection. (b) Hepatic triglyceride (TG) measurement, n = 3. (c) H&E staining of liver at 2-weeks after AAV injection. Arrows indicate small lipid droplets. Scale bar, 25 µm. (d) Western blot of liver lysates from the rescue experiment. (e) Hepatic TG measurement, n = 3. (f) Oil Red O (ORO) staining of liver at 2-weeks after AAV injection. Scale bar, 100 µm. (g) Western blot of total liver lysates. Mice were injected with AAV-GFP or AAV-Hdac3 followed by feeding on normal chow (NC) or high fat diet (HFD) for 6 weeks. Mice were then injected with AAV-Cre and maintained on their previous diet for another 2 weeks before tissue harvest. (h) Hepatic TG measurement in liver from mice described in (g), n = 3–4. (i) H&E staining of liver from mice described in (g). Scale bar, 100 µm. Error bars indicate s.e.m. * P < 0.05 between samples and the GFP controls;