Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Nat Med. 2012 Jun;18(6):934–942. doi: 10.1038/nm.2744

Figure 3. Molecular insulin signaling events remain undisturbed despite lipid accumulation in Hdac3-depleted liver.

Figure 3

(a) Western blot analysis of liver at 2-weeks after AAV injection. Mice were fasted for 18 h. Insulin was injected (2 mU g−1) followed by tissue harvest after 20 min. (b) Western blot analysis of primary hepatocytes isolated from AAV-injected mice after overnight fasting. Cells were challenged with 10 nM insulin for the indicated time and harvested. (c) Hepatic ceramide measured by LC/MS-MS, n = 5. (d) Hepatic DAG measured by gas chromatography, n = 5. (e) Prkce (PKCε) activation assay in liver, n = 6. m/c: membrane versus cytosolic Prkce protein amount. Error bars indicate s.e.m. * P < 0.05.