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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Immunol. 2012 Jul 6;189(4):1937–1945. doi: 10.4049/jimmunol.1200658

Figure 2. Efficacy of mAb RB6-8C5 and 1A8 neutrophil depletion strategies during acute TMEV infection.

Figure 2

Histograms showing the efficacy of neutrophil depletion strategies in representative 7-day TMEV-infected C57BL/6 mice (n=6 per group) using (A,D) normal rat serum, (B,E) RB6-8C5, or (C,F) 1A8. Neutrophil depletion antibodies were administered on days 5, 6, and 7 post-TMEV infection. The CD45hi population was gated and analyzed for the percentages of either GR-1+ or Ly-6G+ cells. (B) RB6-8C5 significantly depleted GR-1+ cells (p<0.001 by Student's t-test), and (F) 1A8 significantly depleted Ly-6G+ cells (p<0.001 by Student's t-test). (G) RB6-8C5 also significantly depleted the total number of GR-1+ cells per 100,000 events (p=0.004 by Mann-Whitney Rank Sum test), and (H) 1A8 significantly depleted the total number of Ly-6G+ cells per 100,000 events (p=0.002 by Mann-Whitney Rank Sum test). (I) RB6-8C5 also caused a significant reduction in CD45+ cells as a percentage of whole brain cells when compared to positive controls treated with normal rat serum (p=0.009 by Student's t-test). Error bars indicate SEM. Shown in A–F are the results of a representative animal in one experiment of two conducted.