Figure 1. IFN-γ and IL-5 responses are optimally detected on day 14.

IFN-γ and IL-5 responses to Rye Grass or Alternaria extract, in three correspondingly allergic donors each, were measured in ELISPOT assays on days 0, 3, 6, 14 and 17. The doses of individual extracts used for in vitro stimulation were chosen on the basis of initial dose titration experiments, and were as shown in Supplemental Table 2.