Figure 2. Comparison of day 6 and day 14 epitope repertoires.
The pattern of Alternaria epitopes identified on days 6 and 14 in two representative donors (D00095 and D00107) were compared. IL-5 or IFN-γ ELISPOT assays were performed, and positive responses defined, as described in the Material and Methods. In terms of IL-5 responses in donor D00095, only peptide 14 was recognized on day 6; by day 14, peptides 11, 13, 14, 15, 16 and 17 were all positive. Similarly, in donor D00107 peptides 14 and 66 gave positive responses on days 6 and 14, while peptides 11, 12, 13, 15, 16, and 17 only gave responses on day 14. For IFN-γ, in both donors positive responses were only seen on day 14.