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Growth of a large Lo domain in the absence of transmembrane pressure for a POPC/PSM/chol membrane. Ordered domains (dark regions) are generated from the right side of bilayer and driven to the pre-existing large domain, revealing that the composition of bilayer during the first 2–3 min following membrane formation is not in equilibrium with the surrounding annulus. Full video of the bilayer stabilization process can be found in SI-1.
