Fig. 6.
A POPC/PSM/chol membrane exhibiting clear phase separation experienced a topological change when 1.63 Pa pressure was applied across the membrane. (a) Domains generated at the bottom of the image migrate and merge with a large domain created during initial membrane formation. (b-c) As perfusion continues, the boundary between ordered and disordered domains becomes increasingly indistinct, until (d) the Lo and Ld regions become fully miscible. Full video of domain behaviors during this experiment can be found in SI-2.