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. 2012 Jul 16;109(31):12811–12816. doi: 10.1073/pnas.1200330109

Fig. 3.

Fig. 3.

Dialysis experiments show that physical interaction of TKS and OAC is not required for OA formation. Recombinant TKS and OAC were assayed in dialysis chambers separated by a 5-kDa cutoff membrane. (A) Assays with TKS and OAC in separate chambers resulted in the formation of HTAL, PDAL, and olivetol in the TKS-containing chamber 1 and HTAL, PDAL, and OA in the OAC-containing chamber 2. (B) Positive control assays with TKS and OAC in chamber 1 and no enzyme in chamber 2 produced large amounts of OA, in addition to HTAL and PDAL. (C) Negative control assays with TKS in chamber 1 and no enzyme in chamber 2 yielded only HTAL, PDAL, and olivetol. Chromatograms were extracted at 270 nm.