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. 2012 Jul 16;109(31):12746–12751. doi: 10.1073/pnas.1115663109

Fig. 1.

Fig. 1.

Determining active biofilm forming DGCs. Each DGC from V. cholerae was induced with 0.1 mM IPTG, and biofilm formation was determined using the MBEC biofilm assay. The ΔvpsL mutant is unable to produce VPS and form significant biofilms. A strain containing an empty vector control and expressing a known active DGC (qrgB) are also indicated. Biofilm fold change is expressed relative to the ΔvpsL mutant. Three biological replicates were performed, and the error bars show the SD.