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Analysis of biofilm formation using flow cytometry. Flow cytometry plots for the induction of VC1599 are shown. The x axis represents side scatter (granularity), and the y axis represents forward scatter (size). The gate P1 brackets the bacterial aggregates in the population. The concentrations (in mM) of IPTG used were: 0.0005 (A); 0.0014 (B); 0.0041 (C); 0.0123 (D); 0.037 (E); 0.11 (F); 0.33 (G); and 1.0 (H).
