Fig. 3.
Correlation of c-di-GMP concentrations with biofilm formation and aphA expression. The amount of biofilm formation is plotted versus the intracellular concentration of c-di-GMP for each of the eight induction states of the seven DGCs that were analyzed (A and B). Each data point represents the average of three independent measurements. Error bars are not shown for clarity. The best-fit line for the entire dataset indicates no significant correlation (A), whereas analysis of the individual DGCs shows significant correlation (B). The r2 values for each DGC in B are indicated in the legend at right. Likewise, aphA expression versus c-di-GMP is shown (C and D). The best-fit line for the complete dataset (C) and individual DGCs (D) are shown. Each DGC is indicated by a different color, as displayed in the legend at right.