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. 2012 Jul 9;109(31):12662–12667. doi: 10.1073/pnas.1209965109

Fig. 5.

Fig. 5.

CRY1 inhibits the forskolin-, PGE2-, and isoproterenol-induced generation of intracellular cAMP, likely by binding to and inhibiting the function of adenylyl cyclase. (A–C) Luciferase assay performed to measure the kinetics of cAMP production after stimulation in the presence of 500 μM of IBMX in 293T cells are shown. Fold stimulation of cAMP production are shown after stimulation with either forskolin, PGE2, or isoproterenol (each 10 μM) in the absence (control vector) or presence of CRY1 expression. Data are mean ± SD (n = 3). (D) CRY1 interacts with adenylyl cyclase. All of the indicated immunoprecipitate and lysate samples analyzed by Western blot for Flag tag (CRY1), adenylyl cyclase III and tubulin are shown. (E) The proposed model showing the likely function of CRY in binding to and suppressing the action of adenylyl cyclase in cAMP production and the pathways activated in the absence of CRY proteins.