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. 2012 Jul 16;109(31):12538–12543. doi: 10.1073/pnas.1209979109

Fig. 3.

Fig. 3.

Close comparison of the propensity for hepatic differentiation between sibling hiPSC lines 201B6 and 201B7. (A) Schematic representation of the modified protocol used for hepatic differentiation. In this protocol, hiPS/ESCs were enzymatically digested into single cells and plated on Matrigel-coated dishes. For endodermal cell induction, the cells were cultivated with activin A and Wnt3a for 7 d. A total of 0.5 mM sodium butyrate (NaB) was supplemented from day 1 for various durations (0–6 d). (B) Albumin secretion level after 21 d of hepatic differentiation. The error bars indicate the SD (n = 3). (C) Percentage of CXCR4-positive cells after 7 d of endodermal differentiation as determined by flow cytometry. Error bars indicate the SD (n = 3). (D) Immunostaining of SOX17 and OCT3/4 after 7 d of endodermal differentiation. NaB was added for 3 d. (Scale bar: 100 μm.)