Fig. 5.
Relative levels of SAP97 and NaV1.5 are significantly increased in hearts of transgenic mice overexpressing Kir2.1. Crude membrane vesicles were prepared from the ventricles of control (WT) and Kir2.1 OE mice. Samples (16 μg/lane) were analyzed by SDS/PAGE and immunoblotted using specific antibodies for SAP97 or NaV1.5, as indicated. (A) Representative immunoblots after detection of protein immunoreactivity with HRP-conjugated secondary antibodies and chemiluminescence (Upper). The corresponding Amido Black nitrocellulose (protein stain) is shown in Lower to show analysis of equal total protein. Protein concentrations were also verified by Lowry assay. (B) Densitometric analysis of data shown in A comparing relative protein levels between WT and Kir2.1 OE mice. Results are expressed as mean signal intensity, and they represent data from three animals for each genotype (N = 3 per genotype, δP < 0.01, mean ± SEM).