Fig. 3.

Effects of FAK-Tyr⁴⁰⁷ mutations on the steady-state levels of BTB regulatory and constituent proteins in Sertoli cells in vitro with a functional TJ–permeability barrier. (A) Immunoblot analysis of selected BTB regulatory (e.g., FAK, c-Src, Eps8, Arp3, N-WASP) and constituent (e.g., occludin, claudin-11, ZO-1, N-cadherin, β-catenin) proteins in Sertoli cells following the transfection of pCI-neo vector only and various FAK constructs. Sertoli cells (0.4 × 10⁶ cells/cm²) cultured for 2 d with an established functional TJ–permeability barrier were transfected with plasmid DNA, and lysates harvested 3 d thereafter were subjected to immunoblotting. Actin served as a protein loading control. (B–E) Histograms summarize immunoblotting results as in A from several independent experiments. Proteins that did not show any significant change were not represented in the histograms. Each data point was normalized against the corresponding actin level and then against the protein level in pCI-neo, which was arbitrarily set as 1. Each bar is a mean ± SD of n = 4–6. *P < 0.05; **P < 0.01 vs. pCI-neo (B and E) or pCI-neo/FAK (C and D); one-way ANOVA followed by the Newman–Keuls test.