Fig. 4.
Effects of FAK-Tyr407 and FAK-Tyr397 mutations on the integrity of the TJ–permeability barrier in Sertoli cells in vitro. Sertoli cells (1.0 × 106 cells/cm2) cultured for 2 d with a functional TJ–permeability barrier were transfected with pCI-neo vector only and various FAK constructs including vector vs. WT (A), WT vs. Y407 mutants (B), WT vs. Y397 mutants (C), and WT vs. Y407, Y397, and double mutants (D). Transepithelial electrical resistance (TER) across the Sertoli cell epithelium was monitored once or twice daily throughout the entire period of culture, and fresh media were replaced thereafter. Data points represent mean ± SD (n = 3–4 replicates in a representative experiment). Similar results were obtained from at least three independent experiments. *P < 0.05; **P < 0.01 vs. pCI-neo/FAK; one-way ANOVA followed by the Newman–Keuls test.