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. 2012 Jul 16;109(31):12817–12821. doi: 10.1073/pnas.1204915109

Fig. 5.

Fig. 5.

TGH contributes to in vivo HYL1–pri-miRNA interaction. (A) Detection of HYL1 protein after immunoprecipitation. Immunoprecipitation was performed with the anti-HYL1 antibody. (B) and (C) association between HYL1 and pri-miR171a and pri-miR167a was impaired in tgh-1. C, Col-0; t, tgh-1. One eighth immunoprecipitates were analyzed by Western blot. Input protein was 2% of total input proteins. The amount of pri-miR167a and pri-miR171a was determined by qRT-PCR and normalized to the input. AtSN1B was used as a negative control (*P < 0.05 and **P < 0.01).