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. Author manuscript; available in PMC: 2012 Aug 4.
Published in final edited form as: J Immunol. 2006 Apr 1;176(7):4208–4220. doi: 10.4049/jimmunol.176.7.4208

FIGURE 3.

FIGURE 3

Small molecules also accelerate dissociation of the high-affinity peptide MBP (85–99) from DR2. Dissociation of fluorescently labeled MBP peptide from DR2 (150 nM) catalyzed by DM (150 nM) was followed by FP in the absence (blue line) and in the presence of different concentrations of small molecules (yellow, orange, and red lines; concentrations indicated in the figure); a molar excess of unlabeled MBP peptide was added to prevent rebinding of the fluorescent peptide. MBP peptide dissociation in the absence of DM and small molecules is also shown in each graph (black line). Peptide dissociation was substantially accelerated by all of the small molecules.