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. Author manuscript; available in PMC: 2014 Mar 1.

Published in final edited form as: Neuropharmacology. 2012 Mar 9;66:122–132. doi: 10.1016/j.neuropharm.2012.03.003

Fig. 7 — Histamine dramatically potentiates the effect of PAMs on mGlu₄-mediated calcium mobilization in cells co-expressing mGlu₄ and H₁ receptors. Traces of calcium transients showing the effects of mGlu₄ PAMs, histamine or the combination of both in potentiating the response of an EC₂₀ concentration of glutamate (1 µM glutamate final) are shown. In these traces, a 10 µM concentration of each mGlu₄ PAM (PHCCC, 4PAM-2, ADX88178 or VU0155041, in A-D respectively; (◇)), 300 nM histamine () or combination of both (◆) were applied in the first add (“Compound/Histamine Add”). After 150 s, 1 µM glutamate (concentration determined based on cAMP experiments shown in Fig. 4A) was applied in the second add (“Glutamate Add”). Calcium responses were measured as the fluorescence ratio, which involves dividing all fluorescence data for each point in the kinetic trace by the fluorescence value obtained in the first baseline sample read, which corrects for differences in dye loading and cell plating.

Inline graphic — Histamine dramatically potentiates the effect of PAMs on mGlu₄-mediated calcium mobilization in cells co-expressing mGlu₄ and H₁ receptors. Traces of calcium transients showing the effects of mGlu₄ PAMs, histamine or the combination of both in potentiating the response of an EC₂₀ concentration of glutamate (1 µM glutamate final) are shown. In these traces, a 10 µM concentration of each mGlu₄ PAM (PHCCC, 4PAM-2, ADX88178 or VU0155041, in A-D respectively; (◇)), 300 nM histamine () or combination of both (◆) were applied in the first add (“Compound/Histamine Add”). After 150 s, 1 µM glutamate (concentration determined based on cAMP experiments shown in Fig. 4A) was applied in the second add (“Glutamate Add”). Calcium responses were measured as the fluorescence ratio, which involves dividing all fluorescence data for each point in the kinetic trace by the fluorescence value obtained in the first baseline sample read, which corrects for differences in dye loading and cell plating.