Fig. 3.

Properties of ES cell lines derived from pluripotin-treated primary outgrowths. a Morphology of newly established mouse ES cell lines. White scale bars: 200 μm, black scale bars: 100 μm. b ES cells are positive for stem cell marker Oct-4. Scale bars: 25 μm. c ES cells form embryoid bodies (EBs) after 10 days of culture in suspension. Scale bars: 200 μm. d Marker analysis after random in vitro differentiation of ES cells. EBs (from c) were plated on gelatinized culture dishes and allowed to attach and spread out for another 10 days in order to differentiate into various cell types, including neurectoderm (Nestin), mesoderm (Smooth muscle actin), skeletal muscle (α-Dystrobrevin) and beating cardiomyocytes (Troponin). Scale bars: 50 μm. e Chimeras generated from ROSA26-AntiLuc and p120ctn^+/− ES cells