Fig. 2.

a, Western blot analysis of kinase phosphorylation in MSC treated with IL-1β. Total cell lysates (30 μg) were separated by SDS-PAGE. Anti-GAPDH was used as loading control. b, MTT assay of MSC cultures treated with IL-1β (25 ng/mL) and IL-6 (20 ng/mL) for 24 h. Values are expressed in fold increase relative to control. c, Cell cycle analysis by flow cytometry of MSC cultures treated with IL-1β (25 ng/mL) for 24 h. (*P < 0.05)