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Leucocyte migration assay of human PMNs using a transwell insert culture system towards different stimuli. Upper chamber were filled with PMNs and lower chambers were seeded with MSC (black bars) or MSC-IKKβ (white bars) treated (dashed and grey bars, respectively) or not with IL-1β. Migrated neutrophils, eosinophils, lymphocytes and monocytes are presented as a mean of three experiments ± S.D. (number of migrated cells/field). *P < 0.05, **P < 0.01, ***P < 0.001
