Table 1.

List of oligonucleotides used for Real time-PCR. Ct and efficiency values are indicated

Gene	Forward primer	Reverse primer	Size (bp)	Ct	SD Ct	Efficencya
ACTB	AGAGCCTCGCCTTTGCCGATCC	CATGCCGGAGCCGTTGTCGAC	101	16.10	0.025	1.971
CCL2	TCTCAGTGCAGAGGCTCGCGA	CCACTTCTGCTTGGGGTCAGCAC	119	17.01	0.006	1.977
CCL3	TTCAGAAGGACACGGGCAGCAGACA	GGAATCTGCCGGGAGGTGTAGCT	216	29.64	0.050	1.862
CCL5	TACATTGCCCGCCCACTGCC	GGGTTGGCACACACTTGGCG	119	23.82	0.052	2.061
CCL7	CAGCTGCTTTCAGCCCCCAGG	GCTTCCCGGGGACAGTGGCTA	148	23.93	0.032	1.923
CCL8	TGGCAGCCACTTTCAGCCCT	GCACAGACCTCCTTGCCCCG	190	27.98	0.167	2.040
CCL20	TCTGCGGCGAATCAGAAGCAGC	TTCATTGGCCAGCTGCCGTGT	110	21.19	0.068	2.16
CLDN1	CCGGGTTGCCCACCTGCAAA	CGTACATGGCCTGGGCGGTC	258	24.49	0.148	1.862
CLDN14	GTCGCTGTGGGCAGGTGGTC	AGCCTCCCCTTCCCAGCCTG	192	30.48	0.093	2.084
CSF2	TGCAGCATCTCTGCACCCGC	AGGCAGGTCGGCTCCTGGAG	176	24.47	0.188	1.708
CSF3	AGACCCATGGCTGGACCTGCC	GTGGCACACTCACTCACCAGCTTC	218	31.56	0.043	2.026
CX3CL1	CTGGCTGGACAGCACCACGG	GCTCCTTCGGGTCGGCACAG	175	25.96	0.087	1.880
CXCL1	AGCCTGCAACATGCCAGCCA	TGTGCACATACATTCCCCTGCCT	94	21.72	0.036	1.848
CXCL10	TGCAAGCCAATTTTGTCCACGTGT	GCAGCCTCTGTGTGGTCCATCC	200	24.10	0.051	1.956
CXCL11	TGTCTTTGCATAGGCCCTGGGGT	AGCCTTGCTTGCTTCGATTTGGGA	164	29.72	0.178	1.821
CXCL3	AATGTAAGGTCCCCCGGACCCC	ACCACCCTGCAGGAAGTGTCAA	199	20.92	0.036	2.023
CXCL5	AATCTCCGCTCCTCCACCCAGT	GCTCTCTCAACACAGCAGCGGC	201	28.49	0.050	1.614
CXCL6	GCACGAGGAAACCAAAGTGCTCTG	GTGCAACGCAGCTCTGTCAGCA	234	26.37	0.071	1.890
ELF3	ATTTAGAGCCGGGTAGGGGAGCG	GTTGCAGCCATGAGGCTACCGGAGT	132	25.68	0.088	1.912
GAPDH	CCCCTCTGCTGATGCCCCCA	TGACCTTGGCCAGGGGTGCT	122	16.79	0.015	1.998
IBSP	GGAGTACGAATACACGGGCGCC	GGTAGCCGGATGCAAAGCCAGA	222	27.12	0.049	2.018
ICAM1	CTGGTCCTGCTCGGGGCTCT	GGGCTGGTCACAGGAGGTGC	126	22.42	0.094	1.638
ICAM4	AATACACTTTGCGCTGCCACGTG	GGCTCCAAGCGAGCATCAGTGT	264	28.16	0.116	2.057
IL11	TGACCCGCTCTCTCCTGGCG	GCACGTGCCGCAGGTAGGAC	192	24.23	0.163	1.803
IL12A	CCCAAAACCTGCTGAGGGCCG	TGGAGGCCAGGCAACTCCCAT	219	29.08	0.047	1.985
IL15	GCTGCTGGAAACCCCTTGCCAT	TAGGTGCTTTGGGCCAACTGGG	214	28.78	0.035	1.899
IL1B	AGGCACAAGGCACAACAGGCT	AACAACTGACGCGGCCTGCC	277	21.12	0.110	1.965
IL23A	TGGCTGTGACCCCCAAGGACTC	TGCCATGGCTGGCTGGGACT	246	27.01	0.053	1.893
IL32	TTTGTGCCAGGAAGACTGCGTGC	GGCTCGACATCACCTGTCCACG	215	24.41	0.078	2.004
IL34	GAACACCACCATGCCCCGGG	CAGCCTGGTGACGTTGGCGA	250	24.59	0.078	1.853
IL6	CATTCTGCCCTCGAGCCCACC	GGCAGCAGGCAACACCAGGA	139	17.08	0.088	1.955
IL7	GCTGCTCGCAAGTTGAGGCAATTT	TTGTTGGTTGGGCTTCACCCAGG	157	26.58	0.213	1.974
IL8	CGTGGCTCTCTTGGCAGCCTTC	TTCCTTGGGGTCCAGACAGAGCTC	229	15.88	0.707	2.005
ITGA9	GCAGTGACCGCTGGCCACTT	GCGCACAAGGAGGAGCCGAA	182	27.63	0.098	1.969
ITGB3	TGCCGCCCTGCTCATCTGGA	TCCTGCAATCGTGGCACAGGC	239	22.43	0.008	2.192
ITGB8	CTAGCGACACTCGGCCCGC	CTGGACCCAGCGCAAGGCAC	292	27.76	0.023	1.850
MMP1	GTGTCTCACAGCTTCCCAGCGAC	GCACTCCACATCTGGGCTGCTTC	238	25.01	0.084	2.006
NOD2	AGGCCTACCCGCAGATGCCA	GTGGGAGAGAGGCTGGCCCA	298	28.04	0.172	2.062
SDC4	CGGAGTCGCCGAGTCGATCC	GGCTCCCAGACCCTGCCCTC	248	20.51	0.030	1.925
SELECTIN	TTGTTCCTGCCAGCAGCTGCC	AGGGCCAGAGACCCGAGGAG	164	17.64	0.085	2.030
SERPINE1	AGGACGAACCGCCAATCGCAAG	ACCCTCACCCCGAAGTCTGAGG	167	25.86	0.034	2.120
TCAM1P	CGAGCTTGGCTGTGGCCTCC	TCTCCGCCATCCCAGCCTCC	225	27.05	0.078	1.856
TLR2	AGGCAGCGAGAAAGCGCAGC	CCCCCAAGACCCACACCATCCA	253	31.23	0.352	2.162
TLR4	CCCTGCGTGGAGGTGGTTCCTA	CTCCCAGGGCTAAACTCTGGATGGG	280	26.45	0.024	2.007
TNF	CCCTCTGGCCCAGGCAGTCA	ATGGGTGGAGGGGCAGCCTT	235	21.58	0.019	2.100
VCAM1	AGGTGACGAATGAGGGGACCACA	CCAGCCTCCAGAGGGCCACT	181	17.31	0.010	2.039

^aPCR efficiency of each PCR was estimated from a serially diluted standard curves obtained from a reference MSC cDNA sample. The cycle number of the crossing point CP vs. Log (dilution factor) were plotted to calculate the slope. The efficiency of each PCR was then calculated with the equation \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ {\hbox{E}} = {1}{0^{[ - {1}/{\rm{slope}}]}} $$\end{document}