Fig. 1.
Macronuclear chromosome copy number and mRNA levels of two genes; Actin (grey), and α-tubulin (white) determined by quantitative PCR: (A) macronuclear chromosome copy number per cell and (B) mRNA expression levels. For each gene, three individual qPCR experiments were performed and triplicates were analyzed (n = 9). Error bars represent the standard deviation. POL, USA-ATCC and USA-SC2 refer to the different strains of C. uncinata used.