. Author manuscript; available in PMC: 2013 Aug 10.

Published in final edited form as: Gene. 2012 May 12;504(2):303–308. doi: 10.1016/j.gene.2012.04.067

Table 2.

Specific primers to target different gene of three strains of Chilodonella uncinata for Quantitative PCR.

Gene	Primer	Primer sequence 5’→ 3	Length (bp)	Tm of Amplicon (°C)
SSU-rDNA	985f	GAGTATGGTCGCAAGGCTGAAAC	162	80.1
SSU-rDNA	1147r	GCACCACCATCCCTCAAATCAAG	162	80.1
Actin	actf	GACTACGAGACCGAGCTCCAGGAGT	160	82.5
Actin	actr	TGGACACCTCCGAAGTCCTTGCCCAA	160	82.5
α-tubulin	504f	CGAGGCCATCTACGATGTTT	212	82.3
α-tubulin	716r	GAGATGATCGGGGCATAAGA	212	82.3
β-tubulin-SP1	615f	CGGCAGATACCTCACTGCCT	154	79.8
β-tubulin-SP1	769r	TTGGAGGGTATCACAGATGGAGC	154	79.8
β-tubulin-SP2	394f	TCCGGAGTCACCTGCTGCTTG	153	81.3
β-tubulin-SP2	547r	GTCAGGGCTCTGTATTGCTGG	153	81.3
β-tubulin-P3	589f	TGCCTCTGCTCTATGAATAACAAG	112	77.3
POL	701r	CGGGTTTGTAATTGCTGAACTGCA	112	77.3
β-tubulin-P3	589f	TGCCTTTGCTCGATGAACAACAAG	109	78.6
USA-ATCC	698r	GTTTGAAGTTGTTGAACCGCGAT	109	78.6
β-tubulin-P3	35f	TCTCGCAGTACGAAGCTGAA	238	81.4
USA-SC2	273r	GCCAGTGTACGAGTGCAAGA	238	81.4
β-tubulin-P2	394f	TCCGGAGTCACCTGCTGTCTC	144	82.5
	538r	TGTATCCTTGTGAGCCGCGA	144	82.5
β-tubulin-P1	615f	CGGCAGATACCTGACTGCTGCT	154	80.9
POL	769r	TCGGCGGTATGTCGCAGATACTGG	154	80.9
β-tubulin-P1	615f	CGGCAGATACCTGACTGCTGCT	154	80.6
USA-ATCC	769r	GCGGTGGGATATCACAGATGCTGC	154	80.6