Fig. 5.

DSBs are reduced in EndoG^−/− B cells. EndoG^+/+ and EndoG^−/− spleen B cells stimulated with LPS and rmIL-4 for 2 days or unstimulated Peyer’s patches B cells were analyzed for DNA break ends. DNA was labeled in situ with biotin-dUTP using TdT (to detect overall DNA cleavages, including both SSBs and DSBs), or biotin-labeled BW linker (biotin-linker) with or without pre-treatment of T4 DNA polymerase and dNTP (to detect total, including 5′-phosphorylated blunt and staggered DSBs, or 5′-phosphorylated blunt DSBs, respectively). Sμ, Sγ1 and Cμ DNA were amplified from serially twofold diluted biotin-labeled DNA and the input DNA by PCR. Data are representative of three independent experiments.