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. Author manuscript; available in PMC: 2012 Dec 29.
Published in final edited form as: Immunity. 2012 Jun 14;36(6):959–973. doi: 10.1016/j.immuni.2012.03.022

Figure 6.

Figure 6

K63 polyubiquitin chains induce the formation of RIG-I tetramer. A. Analytical ultracentrifugation experiments of RIG-I(N) and K63-Ub3, alone (upper panel) or together (bottom panel), were performed. Absorbance at 280 nm and Rayleigh interferometry results were analyzed using SEDPHAT program. Sedimentation coefficient distribution of either RIG-I(N) or K63-Ub3 is shown on the same graph for comparison. B–D. Similar to A., RIG-I(N) and K63 ubiquitin chains of indicated lengths, alone or together, were analyzed by analytical ultracentrifugation. Calculated molar masses and indicated molar ratios are shown for indicated peaks. Results shown are representatives of three experiments.