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. Author manuscript; available in PMC: 2012 Aug 5.
Published in final edited form as: Science. 2010 Jul 30;329(5991):562–565. doi: 10.1126/science.1191880

Figure 2.

Figure 2

Btbd7 expression and regulation by fibronectin. (A–C) In situ hybridization of Btbd7 around the lower sides and bottom of clefts at various stages and magnifications. Open triangles and dotted lines show locations of clefts. Filled triangles highlight Btbd7 expression at the base of a cleft. (D Diagram of Btbd7 transcript pattern. (E) Btbd7 mRNA expression in salivary epithelial cells is induced by fibronectin as quantified by qPCR. (F–H) Exogenous fibronectin (Fn) reduces the organization of the outer layer of columnar epithelial cells; its effects are blocked by Btbd7 siRNA. Epithelia were stained for E-cadherin; traces below each image show basement membrane (white line) and E-cadherin (dashed line). Scale bars: (A–C) 50 µm; (F–H) 10 µm. ***P<0.001. Error bars, s.e.m.