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Humoral immune responses to DEV measured by ELISA. (A) Serum IgG responses to DEV. (B) Serum IgA responses to DEV. Sera were obtained at different time points and assayed by end-point dilution ELISA using DEV as the coating antigen. Numbers on the y-axis represent the means of average OD450 ± standard deviations (n = 3). Statistical significance was determined using a student’s t-test.
