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. Author manuscript; available in PMC: 2012 Aug 5.
Published in final edited form as: Stem Cells. 2009 Apr;27(4):942–948. doi: 10.1002/stem.22

Figure 2. C/EBPβ is involved in the IL-6 expression induced by IFNγ and TNFα.

Figure 2

MSCs were transfected with siRNA specific for C/EBPβ (siC/EBPβ) or a scrambled siRNA control (siControl). After a 72 h incubation, expression levels of C/EBPβ were determined by real time PCR for mRNA (A) and by Western blotting analysis for protein (B). siRNA-transfected MSCs were stimulated with IFNγ, TNFα, or both 48 h after transfection, incubated for an additional 24 h, and IL-6 mRNA assayed by real time PCR (C). Values are given as mean ± SEM of triplicates from a representative of three independent experiments (also applies to Fig. 3–6).