Figure 5. C/EBPβ is involved in the synergistic upregulation of IL-6 by IFNγ in combination with IL-1β or LPS.
MSCs were treated with IFNγ accompanied by IL-1β or LPS for 14 h. C/EBPβ expression was determined by Western blotting analysis (A). MSCs were transfected with C/EBPβ siRNA or control siRNA. 48 h later, these MSCs were stimulated with the indicated combinations of IFNγ and L-1β (B), or IFNγ and LPS (C), incubated for 24 h., and then IL-6 mRNA was assayed by real time PCR.