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. Author manuscript; available in PMC: 2012 Aug 5.
Published in final edited form as: Stem Cells. 2009 Apr;27(4):942–948. doi: 10.1002/stem.22

Figure 6. The role of STAT1 in the synergistic induction of gene expression by IFNγ and TNFα.

Figure 6

MSCs were treated with IFNγ, TNFα, or both for 24 h., and STAT1 expression was determined by real time PCR (A). MSCs transfected with siRNA specific for STAT1 or a scrambled siRNA control were tested after 72 h. for STAT1 expression by real time PCR (B) or by Western blotting analysis (C), or siRNA-transfected MSCs were stimulated with IFNγ, TNFα, or both 48 h after transfection, incubated for an additional 24 h, and the expression of IL-6, iNOS, CCL5 and CXCL1 was determined by real time PCR (D).