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. Author manuscript; available in PMC: 2013 Jan 6.
Published in final edited form as: Cell. 2012 Jul 6;150(1):53–64. doi: 10.1016/j.cell.2012.05.029

Figure 5. Moderate U1 reduction recapitulates isoform switching upon neuronal activation.

Figure 5

Rat PC12 (A) and mouse MN-1 (B) neurons were stimulated with forskolin or forskolin/KCl for 3 hrs or separately transfected with control AMO or increasing amounts of U1 AMO. RT-PCR detecting short and long forms of Homer-1/Vesl-1 and Dab-1 are shown with histograms depicting the ratio of S/L. Grey dotted boxes indicate the region of transcript probed, blue arrows show primer locations, and G6PDH is a loading control.