Fig. 6.

Spa spring water decreased class II MHC expression and impaired pro-inflammatory cytokine production on APCs under TLR3 stimulation. APCs were isolated and stimulated with 20 µg/ml of poly (I : C) for 24 hours. (A) Class II MHC expression was increased under poly (I : C) treatment and significantly reduced by adding spa spring water. Expressed levels were represented by histogram (left) and quantified as MFI (right). Poly (I : C) induced IL-6 (B) and TNF-α (C) were attenuated under spa spring water treatment. The data are expressed as the means±SEM (^*p<0.05) from three independent experiments. APC: antigen presenting cell, TLR: Toll-like receptor, Tx: treatment, MFI: mean fluorescence intensity, IL: interleukin, IFN: interferon, SEM: standard error of the mean.