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. 2011 Oct 25;97(1):121–131. doi: 10.1210/jc.2011-2407

Fig. 5.

Fig. 5.

GLP-1 receptor expression in papillary thyroid carcinoma (subject 41). A, High-magnification photomicrograph of a conventional-type papillary thyroid carcinoma exhibiting papillary structures with fibrovascular cores lined by follicular-derived epithelial cells exhibiting characteristic nuclear changes. These include nuclear enlargement and elongation, overlapping nuclei, fine chromatin, hypochromasia, intranuclear grooves (arrowheads), and intranuclear pseudoinclusions (arrows). A psammoma body is also seen within the circled area. B, A focal area of the same papillary thyroid carcinoma illustrates the specific immunoreactivity for GLP-1 receptor (GLP-1R; NLS1206; red) in some but not all cells of this papillary thyroid carcinoma (DAPI, blue) but no immunoreactivity for calcitonin (green). Scale bar, 50 μm. C–E, An area of adjacent normal (tumor free) thyroid from the same section as B revealed a normal small cluster of C cells immunoreactive for calcitonin (green, C) that were not labeled for GLP-1R (NLS1206; red, D). Adjacent thyroid follicular cells apparent in the merged image (DAPI, blue, E) were negative for both calcitonin and GLP-1R. The specific immunoreactivity of these C cells in the same section as papillary thyroid carcinoma cells in A and B serves as a positive control for calcitonin labeling and assures that GLP-1R (B) is indeed present in papillary thyroid carcinoma cells and not a mixture of papillary thyroid carcinoma and C cells. Scale bar, 50 μm.